osteoblast like 7f2 mouse cells  (ATCC)

Journal: Pharmaceuticals

Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

doi: 10.3390/ph15040490

Figure Lengend Snippet: In vitro uptake of DiI-labeled liposomes in 7F2 osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).

Article Snippet: Mouse osteoblast-like cells (7F2) and mouse macrophage cells (Raw264.7) were obtained from Bioresource Collection and Research Center (BCRC), Taiwan.

Techniques: In Vitro, Labeling, Liposomes, Microscopy, Cell Culture, Fluorescence, Staining

Journal: Pharmaceuticals

Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

doi: 10.3390/ph15040490

Figure Lengend Snippet: The effect of astaxanthin extract and asta-loaded liposomes on cell viability of 7F2 osteoblasts. 7F2 osteoblasts were treated with ( A ) astaxanthin extract and ( B ) empty and asta-loaded liposomes for 24 h. Cell viabilities of 7F2 osteoblasts were measured by MTT assay. The data are shown as the means ± standard deviation. (* p < 0.05 related to control; + p < 0.05 related to 2% ETOH; # p < 0.05 related to liposomes).

Article Snippet: Mouse osteoblast-like cells (7F2) and mouse macrophage cells (Raw264.7) were obtained from Bioresource Collection and Research Center (BCRC), Taiwan.

Techniques: Liposomes, MTT Assay, Standard Deviation, Control

Journal: Pharmaceuticals

Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

doi: 10.3390/ph15040490

Figure Lengend Snippet: The effect of asta-loaded liposomes on 7F2 osteoblast differentiation and mineralization. 7F2 osteoblasts were treated with 50 μg/mL of ascorbic and 10 mM β-glycerophosphate to induce osteoblast differentiation and mineralization. ( A ) Cells were then incubated in the presence or absence of asta-loaded liposomes for 1, 4 and 7 days. Results are revealed as a ratio with mean ± standard deviation ( n = 3). ( B ) ARS staining of calcium deposits on days 1, 7 and 14 (×100 magnification, scale bar = 200 nm). ( C ) Quantification of calcium deposits on days 1, 7 and 14. Calcium deposition was quantified by dissolving ARS aggregates into 10% cetylpyridinium chloride and evaluating the absorbance at 560 nm. Results are revealed as a ratio with mean ± standard deviation ( n = 3) (* p < 0.05 related to control group of the same day, # p < 0.05 related to MM of the same day, + p < 0.05 related to empty liposome treatment of the same day).

Article Snippet: Mouse osteoblast-like cells (7F2) and mouse macrophage cells (Raw264.7) were obtained from Bioresource Collection and Research Center (BCRC), Taiwan.

Techniques: Liposomes, Incubation, Standard Deviation, Staining, Control